Cloning of bovine peroxiredoxins-gene expression in bovine tissues and amino acid sequence comparison with rat, mouse and primate peroxiredoxins

Comp Biochem Physiol B Biochem Mol Biol. 2003 Dec;136(4):943-55. doi: 10.1016/s1096-4959(03)00290-2.

Abstract

The peroxiredoxin (PRDX) family is a recently identified family of peroxidases found in organisms ranging from bacteria to mammals. In mammals, six PRDX isoforms have been characterized in human (Homo sapiens), rat (Rattus norvegicus) and mouse (Mus musculus). PRDXs are cytosolic, secreted or targeted to organelles such as peroxisomes, mitochondria and the nucleus. Some PRDXs are synthesized as larger precursor proteins with a presequence that is cleaved to produce the mature form. To study the expression of the six PRDXs in bovine (Bos taurus), we first cloned cDNAs coding for PRDX1, PRDX2, PRDX4 and PRDX5. PRDX3 and PRDX6 had previously been cloned and characterized in bovine. The comparison of bovine PRDXs with their rat, mouse and primate orthologues reveals a minimum of 95% similarity of mature proteins. Even though mitochondrial or export signal presequences are normally less conserved, the unprocessed proteins still present a minimum of 84% similarity. Nevertheless, a major divergence lies at the N-terminus of bovine PRDX2, where a Cys-Val-Cys motif was identified. The expression of the six PRDXs in 22 bovine tissues has been studied by RT-PCR. Our results point out the ubiquity of the different PRDX transcripts in bovine tissues. The important conservation of the different PRDXs, the multiple processes they have been associated with, as well as the ubiquity of all the members of the family analyzed in this study for the first time altogether, suggest that they play a major role in the basal metabolism of mammalian cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cattle / genetics*
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Gene Expression Profiling*
  • Isoenzymes / genetics
  • Liver / enzymology
  • Mice
  • Molecular Sequence Data
  • Organ Specificity
  • Peroxidases / chemistry*
  • Peroxidases / genetics*
  • Peroxiredoxin VI
  • Peroxiredoxins
  • Phylogeny
  • Primates*
  • Rats
  • Sequence Alignment
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • Isoenzymes
  • Peroxidases
  • PRDX1 protein, human
  • PRDX5 protein, human
  • PRDX6 protein, human
  • Peroxiredoxin VI
  • Peroxiredoxins
  • Prdx5 protein, mouse
  • Prdx6 protein, mouse
  • Prdx6 protein, rat