Evaluation of a PCR-based universal heteroduplex generator assay as a tool for rapid detection of multidrug-resistant Mycobacterium tuberculosis in Peru

J Clin Microbiol. 2003 Dec;41(12):5774-7. doi: 10.1128/jcm.41.12.5774-5777.2003.

Abstract

Multidrug-resistant tuberculosis is an increasing health problem worldwide, especially in developing countries. The PCR-UHG-Rif assay, which detects mutations within the rpoB gene associated with rifampin resistance, was evaluated for its ability and reliability to detect and identify drug-resistant Mycobacterium tuberculosis in a developing country where tuberculosis is highly endemic.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antitubercular Agents / pharmacology
  • Antitubercular Agents / therapeutic use
  • DNA-Directed RNA Polymerases / genetics
  • Drug Resistance, Multiple / genetics*
  • Humans
  • Microbial Sensitivity Tests
  • Mycobacterium tuberculosis / drug effects
  • Mycobacterium tuberculosis / genetics*
  • Mycobacterium tuberculosis / isolation & purification
  • Peru
  • Polymerase Chain Reaction / methods*
  • Predictive Value of Tests
  • Rifampin / pharmacology
  • Rifampin / therapeutic use
  • Sensitivity and Specificity
  • Tuberculosis / drug therapy

Substances

  • Antitubercular Agents
  • DNA-Directed RNA Polymerases
  • RNA polymerase beta subunit
  • Rifampin