A protein encoded by a member of the multicopy Ssty gene family located on the long arm of the mouse Y chromosome is expressed during sperm development

Genomics. 2004 Jan;83(1):140-7. doi: 10.1016/s0888-7543(03)00216-7.


Multicopy Y-chromosomal genes in human and mouse have been postulated to play a role in spermatogenesis. The mouse Y long arm (Yq) carries hundreds of supposedly intronless copies of Ssty, for which no protein has hitherto been identified; mice lacking Yq are sterile with grossly abnormal sperm. We have now identified an Ssty-encoded protein (Ssty1) that is expressed in spermatids. The protein is absent from spermatids of mice that lack Yq, but is not reduced in mice with a two-thirds reduction of Ssty copies, implying that most do not produce this protein. Furthermore, no protein was produced by a strongly transcribed intronless Ssty transgene, raising doubts as to the protein-encoding potential of these intronless genes. We have now identified an intron-containing copy that is also present in multiple copies on Yq. One or more intron-containing copies are retained in the Ssty-deficient mice and may be the source of the Ssty1 protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibody Affinity
  • Base Sequence
  • Blotting, Western
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Introns / genetics
  • Male
  • Mice
  • Molecular Sequence Data
  • Multigene Family / genetics*
  • Proteins / genetics*
  • Proteins / immunology
  • Proteins / metabolism
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Spermatids / metabolism
  • Spermatogenesis / genetics*
  • Y Chromosome / genetics*


  • DNA, Complementary
  • Proteins
  • Ssty1 protein, mouse