Meal modulation of circulating interleukin 18 and adiponectin concentrations in healthy subjects and in patients with type 2 diabetes mellitus

Am J Clin Nutr. 2003 Dec;78(6):1135-40. doi: 10.1093/ajcn/78.6.1135.


Background: A single high-fat meal induces endothelial activation, which is associated with increased serum concentrations of inflammatory cytokines.

Objective: We compared the effect of 3 different meals on circulating concentrations of interleukin 8 (IL-8), interleukin 18 (IL-18), and adiponectin in healthy subjects and in patients with type 2 diabetes mellitus.

Design: Thirty patients with newly diagnosed type 2 diabetes and 30 matched, nondiabetic subjects received the following 3 isoenergetic (780 kcal) meals separated by 1-wk intervals: a high-fat meal; a high-carbohydrate, low-fiber (4.5 g) meal; and a high-carbohydrate, high-fiber meal in which refined-wheat flour was replaced with whole-wheat flour (16.8 g). We analyzed serum glucose and lipid variables and serum IL-8, IL-18, and adiponectin concentrations at baseline and at 2 and 4 h after ingestion of the meals.

Results: Compared with nondiabetic subjects, diabetic patients had higher fasting IL-8 (P < 0.05) and IL-18 (P < 0.01) concentrations and lower adiponectin concentrations (P < 0.01) at baseline. In both nondiabetic and diabetic subjects, IL-18 concentrations increased and adiponectin concentrations decreased (P < 0.05) from baseline concentrations after consumption of the high-fat meal. After consumption of the high-carbohydrate, high-fiber meal, serum IL-18 concentrations decreased from baseline concentrations (P < 0.05) in both nondiabetic and diabetic subjects; adiponectin concentrations decreased after the high-carbohydrate, low-fiber meal in diabetic patients. IL-8 concentrations did not change significantly after consumption of any of the 3 meals.

Conclusions: This study provides evidence that circulating IL-18 and adiponectin concentrations are modulated by familiar foodstuffs in humans. Meal modulation of cytokines involved in atherogenesis may represent a safe strategy for ameliorating atherogenetic inflammatory activity in diabetic patients.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adiponectin
  • Adult
  • Blood Glucose / analysis
  • Coronary Artery Disease / blood
  • Coronary Artery Disease / diet therapy
  • Coronary Artery Disease / metabolism
  • Cross-Over Studies
  • Diabetes Mellitus, Type 2 / blood*
  • Diabetes Mellitus, Type 2 / diet therapy
  • Diabetes Mellitus, Type 2 / metabolism
  • Dietary Carbohydrates / administration & dosage*
  • Dietary Fats / administration & dosage*
  • Dietary Fiber / administration & dosage*
  • Female
  • Humans
  • Intercellular Signaling Peptides and Proteins*
  • Interleukin-18 / biosynthesis
  • Interleukin-18 / blood*
  • Interleukin-8 / biosynthesis
  • Interleukin-8 / blood
  • Male
  • Proteins / metabolism*


  • Adiponectin
  • Blood Glucose
  • Dietary Carbohydrates
  • Dietary Fats
  • Dietary Fiber
  • Intercellular Signaling Peptides and Proteins
  • Interleukin-18
  • Interleukin-8
  • Proteins