Functional activity of eukaryotic signal sequences in Escherichia coli: the ovalbumin family of serine protease inhibitors

J Mol Biol. 2004 Jan 9;335(2):437-53. doi: 10.1016/j.jmb.2003.10.076.

Abstract

It is widely assumed that the functional activity of signal sequences has been conserved throughout evolution, at least between Gram-negative bacteria and eukaryotes. The ovalbumin family of serine protease inhibitors (serpins) provides a unique tool to test this assumption, since individual members can be secreted (ovalbumin), cytosolic (leukocyte elastase inhibitor, LEI), or targeted to both compartments (plasminogen activator inhibitor 2, PAI-2). The facultative secretion of PAI-2 is mediated by a signal sequence proposed to be inefficient by design. We show here that the same internal domain that promotes an inefficient translocation of murine PAI-2 in mammalian cells is a weak signal sequence in Escherichia coli. In contrast, the ovalbumin signal sequence is much more efficient, whereas the corresponding sequence elements from LEI, maspin and PI-10 are entirely devoid of signal sequence activity in E.coli. Mutations that improve the activity of the PAI-2 signal sequence and that convert the N-terminal regions of maspin and PI-10 into efficient signal sequences have been characterized. Taken together, these results indicate that several structural features contribute to the weak activity of the PAI-2 signal sequence and provide new insights into the plasticity of the "hydrophobic core" of signal sequences. High-level expression of two chimeric proteins containing the PAI-2 signal sequence is toxic, and the reduced viability is accompanied by a rapid decrease in the membrane proton motive force, in ATP levels and in translation. In unc- cells, which lack the F0F1 ATP-synthase, the chimeric proteins retain their toxicity and their expression only affected the proton motive force. Thus, the properties of these toxic signal sequences offer a new tool to dissect the interactions of signal sequences with the protein export machinery.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Triphosphatases / metabolism
  • Adenosine Triphosphate / metabolism
  • Alkaline Phosphatase / metabolism
  • Amino Acid Sequence
  • Animals
  • Chickens
  • Consensus Sequence
  • Cyclin-Dependent Kinases / genetics
  • Cyclin-Dependent Kinases / metabolism
  • Escherichia coli / metabolism
  • Escherichia coli Proteins
  • Female
  • Genes, Tumor Suppressor
  • Humans
  • Mice
  • Molecular Sequence Data
  • Mutation
  • Ovalbumin / physiology*
  • Plasmids
  • Plasminogen Activator Inhibitor 2 / physiology*
  • Protein Sorting Signals / physiology*
  • Protein Structure, Tertiary
  • Protein Transport
  • Proteins / genetics
  • Proteins / metabolism*
  • Proton-Motive Force
  • Recombinant Fusion Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Serpins / genetics
  • Serpins / metabolism*

Substances

  • Escherichia coli Proteins
  • Plasminogen Activator Inhibitor 2
  • Protein Sorting Signals
  • Proteins
  • Recombinant Fusion Proteins
  • SERPIN-B5
  • SERPINB10 protein, human
  • Serpins
  • Adenosine Triphosphate
  • Ovalbumin
  • Cyclin-Dependent Kinases
  • Alkaline Phosphatase
  • phoA protein, E coli
  • Adenosine Triphosphatases