Molecular cloning and characterization of a cDNA encoding ent-cassa-12,15-diene synthase, a putative diterpenoid phytoalexin biosynthetic enzyme, from suspension-cultured rice cells treated with a chitin elicitor

Plant J. 2004 Jan;37(1):1-8. doi: 10.1046/j.1365-313x.2003.01926.x.

Abstract

We have isolated and characterized a cDNA encoding a novel diterpene cyclase, OsDTC1, from suspension-cultured rice cells treated with a chitin elicitor. OsDTC1 functions as ent-cassa-12,15-diene synthase, which is considered to play a key role in the biosynthesis of (-)-phytocassanes recently isolated as rice diterpenoid phytoalexins. The expression of OsDTC1 mRNA was also confirmed in ultraviolet (UV)-irradiated rice leaves. In addition, we identified ent-cassa-12,15-diene, a putative diterpene hydrocarbon precursor of (-)-phytocassanes, as an endogenous compound in the chitin-elicited suspension-cultured rice cells and the UV-irradiated rice leaves. The OsDTC1 cDNA isolated here will be a useful tool to investigate the regulatory mechanisms of the biosynthesis of (-)-phytocassanes in rice.

MeSH terms

  • Amino Acid Sequence
  • Cells, Cultured
  • Chitin / analogs & derivatives
  • Chitin / pharmacology
  • Cloning, Molecular
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • Diterpenes / metabolism*
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Gene Expression Regulation, Enzymologic
  • Gene Expression Regulation, Plant
  • Isomerases / genetics*
  • Isomerases / metabolism
  • Molecular Sequence Data
  • Oligosaccharides / pharmacology*
  • Oryza / enzymology
  • Oryza / genetics*
  • Oryza / metabolism
  • Plant Extracts / biosynthesis*
  • Plant Leaves / enzymology
  • Plant Leaves / genetics
  • Plant Leaves / radiation effects
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Sesquiterpenes
  • Terpenes
  • Ultraviolet Rays

Substances

  • DNA, Complementary
  • Diterpenes
  • Oligosaccharides
  • Plant Extracts
  • Plant Proteins
  • RNA, Messenger
  • Sesquiterpenes
  • Terpenes
  • Chitin
  • phytoalexins
  • DTC1 protein, Oryza sativa
  • Isomerases

Associated data

  • GENBANK/AB089272