EIN3-dependent Regulation of Plant Ethylene Hormone Signaling by Two Arabidopsis F Box Proteins: EBF1 and EBF2

Cell. 2003 Dec 12;115(6):679-89. doi: 10.1016/s0092-8674(03)00968-1.

Abstract

The plant hormone ethylene regulates a wide range of developmental processes and the response of plants to stress and pathogens. Genetic studies in Arabidopsis led to a partial elucidation of the mechanisms of ethylene action. Ethylene signal transduction initiates with ethylene binding at a family of ethylene receptors and terminates in a transcription cascade involving the EIN3/EIL and ERF families of plant-specific transcription factors. Here, we identify two Arabidopsis F box proteins called EBF1 and EBF2 that interact physically with EIN3/EIL transcription factors. EBF1 overexpression results in plants insensitive to ethylene. In contrast, plants carrying the ebf1 and ebf2 mutations display a constitutive ethylene response and accumulate the EIN3 protein in the absence of the hormone. Our work places EBF1 and EBF2 within the genetic framework of the ethylene-response pathway and supports a model in which ethylene action depends on EIN3 protein stabilization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / drug effects
  • Arabidopsis / genetics
  • Arabidopsis / growth & development*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / isolation & purification*
  • Arabidopsis Proteins / metabolism
  • Cell Nucleus / genetics
  • Cell Nucleus / metabolism
  • DNA, Complementary / analysis
  • DNA, Complementary / genetics
  • DNA-Binding Proteins
  • Ethylenes / metabolism*
  • Ethylenes / pharmacology
  • F-Box Proteins / genetics
  • F-Box Proteins / isolation & purification*
  • Gene Expression Regulation, Plant / drug effects
  • Gene Expression Regulation, Plant / genetics
  • Molecular Sequence Data
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Phenotype
  • Plant Proteins
  • Receptors, Cell Surface / metabolism
  • SKP Cullin F-Box Protein Ligases / genetics
  • SKP Cullin F-Box Protein Ligases / isolation & purification*
  • Seedlings / drug effects
  • Seedlings / genetics
  • Seedlings / growth & development
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Signal Transduction / drug effects
  • Signal Transduction / genetics
  • Transcription Factors*

Substances

  • Arabidopsis Proteins
  • DNA, Complementary
  • DNA-Binding Proteins
  • EBF1 protein, Arabidopsis
  • EBF2 protein, Arabidopsis
  • EIN2 protein, Arabidopsis
  • EIN3 protein, Arabidopsis
  • Ethylenes
  • F-Box Proteins
  • Nuclear Proteins
  • Plant Proteins
  • Receptors, Cell Surface
  • Transcription Factors
  • ethylene-responsive element binding protein
  • ethylene
  • SKP Cullin F-Box Protein Ligases