Chromium (III) enhanced diamine silver staining of proteins and DNA in gels

Biotechnol Lett. 2003 Nov;25(21):1801-4. doi: 10.1023/a:1026280519327.

Abstract

Chromium (III) enhanced the sensitivities of diamine silver staining of four proteins between 6- and 50-fold over that of the Coomassie Brilliant Blue (CBB)-chromium modified thiosulfate-silver staining method (Zhou et al. Biotechnology Letters, 2002, 24: 1561-1567). Using six dsDNA fragments, the detection limits of this new method was 10 to 30 pg per band, being 10- to 25-fold more sensitive than previous methods.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Chromium*
  • DNA / analysis*
  • DNA / chemistry
  • Diamines*
  • Electrophoresis, Polyacrylamide Gel / methods*
  • Proteins / analysis*
  • Proteins / chemistry
  • Reproducibility of Results
  • Rosaniline Dyes*
  • Sensitivity and Specificity
  • Silver*
  • Staining and Labeling / methods*

Substances

  • Diamines
  • Proteins
  • Rosaniline Dyes
  • Chromium
  • Silver
  • DNA
  • coomassie Brilliant Blue