Abstract
The Caenorhabditis elegans excretory canal is composed of a single elongated and branched cell that is tunneled by an inner lumen of apical character. Loss of the exc-4 gene causes a cystic enlargement of this intracellular tube. exc-4 encodes a member of the chloride intracellular channel (CLIC) family of proteins. EXC-4 protein localizes to various tubular membranes in distinct cell types, including the lumenal membrane of the excretory tubes. A conserved 55-amino acid domain enables EXC-4 translocation from the cytosol to the lumenal membrane. The tubular architecture of this membrane requires EXC-4 for both its formation and maintenance.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Animals, Genetically Modified
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Caenorhabditis elegans / cytology
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Caenorhabditis elegans / embryology*
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Caenorhabditis elegans / growth & development
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Caenorhabditis elegans / physiology*
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Caenorhabditis elegans Proteins / chemistry
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Caenorhabditis elegans Proteins / genetics
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Caenorhabditis elegans Proteins / metabolism*
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Cell Membrane / metabolism*
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Chloride Channels / chemistry
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Chloride Channels / genetics
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Chloride Channels / metabolism*
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Cytoplasm / metabolism
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Epithelial Cells / metabolism
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Gene Expression
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Genes, Reporter
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Green Fluorescent Proteins
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Hot Temperature
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Humans
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Intracellular Membranes / metabolism*
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Luminescent Proteins
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Molecular Sequence Data
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Morphogenesis
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Mutation
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Pinocytosis
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Promoter Regions, Genetic
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Protein Structure, Secondary
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Protein Structure, Tertiary
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Recombinant Fusion Proteins / metabolism
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Vacuoles / metabolism*
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Vacuoles / ultrastructure
Substances
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CLIC4 protein, human
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Caenorhabditis elegans Proteins
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Chloride Channels
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EXC-4 protein, C elegans
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Luminescent Proteins
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Recombinant Fusion Proteins
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Green Fluorescent Proteins