Presence and coding properties of 2'-O-methyl-5-carbamoylmethyluridine (ncm5Um) in the wobble position of the anticodon of tRNA(Leu) (U*AA) from brewer's yeast

FEBS Lett. 1992 Dec 21;314(3):381-5. doi: 10.1016/0014-5793(92)81510-s.

Abstract

The unknown modified nucleoside U* has been isolated by enzymatic and HPLC protocols from tRNA(Leu) (U*AA) recently discovered in brewer's yeast. The pure U* nucleoside has been characterized by electron impact mass spectroscopy, and comparison of its chromatographic and UV-absorption properties with those of appropriate synthetic compounds. The structure of U* was established as 2'-O-methyl-5-carbamoylmethyluridine (ncm5Um). The yeast tRNA(Leu) (U*AA) is the only tRNA so far sequenced which has been shown to contain ncm5Um. The location of such a modified uridine at the first position of the anticodon restricts the decoding property to A of the leucine UUA codon.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anticodon*
  • Chromatography, High Pressure Liquid
  • Fungal Proteins / biosynthesis
  • Mass Spectrometry
  • Molecular Structure
  • RNA, Fungal / genetics
  • RNA, Transfer, Leu / genetics*
  • Saccharomyces cerevisiae / genetics*
  • Spectrophotometry, Ultraviolet
  • Uridine / analogs & derivatives*
  • Uridine / analysis
  • Uridine / chemistry
  • Uridine / genetics

Substances

  • Anticodon
  • Fungal Proteins
  • RNA, Fungal
  • RNA, Transfer, Leu
  • O(2)'-methyl-5-carbamoylmethyluridine
  • Uridine