A mechanism for the regulation of pre-mRNA 3' processing by human cleavage factor Im

Mol Cell. 2003 Dec;12(6):1467-76. doi: 10.1016/s1097-2765(03)00453-2.


Human cleavage factor I(m) (CFI(m)) is a heterodimeric RNA binding protein complex that functions at an early step in the assembly of the pre-mRNA 3' processing complex. In this report we show that CFI(m) can stimulate both cleavage and poly(A) addition, and can act to suppress poly(A) site cleavage in a sequence-dependent manner. Elevated levels of CFI(m) suppressed cleavage at the primary poly(A) site of the pre-mRNA encoding the 68 kDa subunit of CFI(m). CFI(m)-mediated suppression of poly(A) site cleavage was dependent upon the presence of three copies of an RNA element initially identified by CFI(m)-SELEX. These data provide evidence for a mechanism for the regulation of poly(A) site selection by a basal pre-mRNA 3' processing factor.

MeSH terms

  • 3' Untranslated Regions / metabolism*
  • Base Sequence
  • Humans
  • Molecular Sequence Data
  • Polyadenylation
  • Protein Subunits / metabolism
  • RNA Processing, Post-Transcriptional*
  • RNA, Messenger / metabolism*
  • RNA-Binding Proteins / metabolism*
  • mRNA Cleavage and Polyadenylation Factors / metabolism*


  • 3' Untranslated Regions
  • Protein Subunits
  • RNA, Messenger
  • RNA-Binding Proteins
  • cleavage factor Im, human
  • mRNA Cleavage and Polyadenylation Factors