Description of a Putative Type I Secretion System in Legionella Pneumophila

Int J Med Microbiol. 2003 Nov;293(5):349-58. doi: 10.1078/1438-4221-00276.


Here we describe the first putative type I secretion system of L. pneumophila. The lssXYZABD locus was found to be present in all L. pneumophila strains tested so far. The LssB and LssD proteins are homologues to the proteins of type I secretion systems of Vibrio cholerae and Salmonella typhi. The expression of the gene locus was analysed by RT-PCR. Three polycistronic transcripts could be identified. Comparison of the lss locus of L. pneumophila Philadelphia I with that of L. pneumophila Corby revealed protein identities of 97 to 99%, except for LssE proteins that were only 78.6% identical. Computer analysis of the amino acid sequence of LssE identified it as a putative signalling protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Cloning, Molecular
  • Gene Expression Regulation, Bacterial
  • Humans
  • Legionella pneumophila / genetics
  • Legionella pneumophila / metabolism
  • Legionella pneumophila / physiology*
  • Molecular Sequence Data
  • Protein Sorting Signals / genetics
  • RNA, Bacterial / genetics
  • RNA, Bacterial / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Alignment


  • Protein Sorting Signals
  • RNA, Bacterial