Chlamydia pneumoniae induces the expression of inhibitor of apoptosis 2 (c-IAP2) in a human monocytic cell line by an NF-kappaB-dependent pathway

Int J Med Microbiol. 2003 Nov;293(5):377-81. doi: 10.1078/1438-4221-00274.


Members of the inhibitor of apoptosis protein family are important factors that regulate apoptotic cell death. As demonstrated both by RT-PCR and Western Blot analysis C. pneumoniae infection of the human monocytic cell line Mono Mac 6 induces the expression of mRNA and protein of the cellular inhibitor of apoptosis 2 (c-IAP2). Blocking NF-kappaB DNA-binding activity by the proteasome inhibitor MG-132 results in decrease of C. pneumoniae-induced c-IAP2 expression. Therefore, C. pneumoniae may exploit the NF-kappaB pathway to induce expression of an antiapoptotic host cell protein that may contribute to intracellular survival of the pathogen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / physiology*
  • Blotting, Western
  • Chlamydia Infections / genetics
  • Chlamydia Infections / metabolism*
  • Chlamydophila pneumoniae*
  • Cysteine Proteinase Inhibitors / pharmacology
  • Electrophoretic Mobility Shift Assay
  • Gene Expression Regulation
  • Humans
  • Leupeptins / pharmacology
  • Monocytes / metabolism*
  • NF-kappa B / physiology*
  • Protein Biosynthesis*
  • Proteins / genetics
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Reverse Transcriptase Polymerase Chain Reaction


  • Cysteine Proteinase Inhibitors
  • Leupeptins
  • NF-kappa B
  • Proteins
  • RNA, Messenger
  • benzyloxycarbonylleucyl-leucyl-leucine aldehyde