Defined conditions for neural commitment and differentiation

Methods Enzymol. 2003;365:327-41. doi: 10.1016/s0076-6879(03)65023-8.

Abstract

The efficiency of monolayer differentiation establishes that commitment of ES cells to a neural fate needs neither multicellular aggregation nor extrinsic inducers. The entire process by which pluripotent ES cells acquire neural specification can be visualized and recorded at the level of individual colonies. Furthermore this simple culture system is amenable to cellular and molecular dissection, promising to yield new insights into the mechanism underlying neural determination in mammals and perhaps to deliver the goal of "directed" homogeneous differentiation of ES cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques / methods*
  • Cell Death
  • Cell Differentiation
  • Cell Lineage
  • Cell Separation
  • Cells, Cultured
  • Culture Media / pharmacology
  • Culture Media, Serum-Free / pharmacology
  • DNA-Binding Proteins / metabolism
  • Flow Cytometry
  • Green Fluorescent Proteins
  • HMGB Proteins
  • High Mobility Group Proteins / metabolism
  • Luminescent Proteins / metabolism
  • Mice
  • Microscopy, Phase-Contrast
  • Neurons / cytology*
  • Nuclear Proteins / metabolism
  • SOXB1 Transcription Factors
  • Transcription Factors

Substances

  • Culture Media
  • Culture Media, Serum-Free
  • DNA-Binding Proteins
  • HMGB Proteins
  • High Mobility Group Proteins
  • Luminescent Proteins
  • Nuclear Proteins
  • SOXB1 Transcription Factors
  • Sox1 protein, mouse
  • Sox2 protein, mouse
  • Transcription Factors
  • Green Fluorescent Proteins