Overproduction of PU.1 in mast cell progenitors: its effect on monocyte- and mast cell-specific gene expression

Biochem Biophys Res Commun. 2004 Jan 16;313(3):516-21. doi: 10.1016/j.bbrc.2003.11.145.


The Ets family transcription factor PU.1 is required for development of various lymphoid and myeloid cell lineages, and regulates the expression of several genes in a cell type-specific manner. Mouse bone marrow-derived hematopoietic progenitor cells are programmed to differentiate into mast cells, when the cells are maintained in the presence of pokeweed mitogen-stimulated spleen-conditioned medium. However, by retroviral introduction of PU.1 cDNA, the progenitor cells expressed MHC class II, CD11b, CD11c, and F4/80, and acquired the ability to stimulate T cells. Furthermore, PU.1-overproducing cells exhibited the morphology, in part, similar to that of monocyte. These results indicate that the mast cell progenitors still have the ability to express monocyte-specific genes by increased expression of PU.1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen Presentation
  • Antigens, Differentiation / biosynthesis
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / metabolism
  • CD11b Antigen / biosynthesis
  • CD11c Antigen / biosynthesis
  • CD4-Positive T-Lymphocytes / metabolism
  • Cell Line
  • Cell Lineage
  • DNA, Complementary / metabolism
  • Flow Cytometry
  • Gene Expression*
  • Mast Cells / metabolism*
  • Mice
  • Mice, Inbred BALB C
  • Monocytes / metabolism*
  • Proto-Oncogene Proteins / biosynthesis*
  • Retroviridae / genetics
  • Stem Cells / metabolism*
  • T-Lymphocytes / metabolism
  • Trans-Activators / biosynthesis*


  • Antigens, Differentiation
  • CD11b Antigen
  • CD11c Antigen
  • DNA, Complementary
  • Proto-Oncogene Proteins
  • Trans-Activators
  • monocyte-macrophage differentiation antigen
  • proto-oncogene protein Spi-1