Establishment of cell lines from a primary hepatocellular carcinoma and its metastatis

Cancer Genet Cytogenet. 2004 Jan 1;148(1):80-4. doi: 10.1016/s0165-4608(03)00206-1.


Hepatocellular carcinoma (HCC) is one of the most common malignancies in the world with a very poor prognosis that has been associated with tumor metastasis. The molecular mechanism of HCC metastasis is still unclear. In this study, we established cell lines from a primary tumor (H2-P) and its metastasis (H2-M). G-banding karyotyping, comparative genomic hybridization, and fluorescence in situ hybridization were applied to study these two cell lines and the results demonstrated that they are of the same origin. These cell lines provide a very useful tool to identify genetic alterations associated with HCC metastasis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Carcinoma, Hepatocellular / genetics
  • Carcinoma, Hepatocellular / pathology*
  • Carcinoma, Hepatocellular / secondary
  • Chromosome Aberrations*
  • Chromosome Banding
  • Humans
  • In Situ Hybridization, Fluorescence
  • Liver Neoplasms / genetics
  • Liver Neoplasms / pathology*
  • Male
  • Nucleic Acid Hybridization
  • Tumor Cells, Cultured