Activity-inducible protein Homer1a/Vesl-1S promotes redistribution of postsynaptic protein Homer1c/Vesl-1L in cultured rat hippocampal neurons

Neurosci Lett. 2004 Jan 9;354(2):143-7. doi: 10.1016/j.neulet.2003.09.082.

Abstract

In cultured rat hippocampal neurons, overexpression of Homer1a/Vesl-1S, an inducible protein upregulated by seizure or long-term potentiation, caused a reduction of punctate distribution of a postsynaptic protein Homer1c/Vesl-1L, without significant decrease in its total amount. Clusters of F-actin were also decreased. Treatments of cells with BDNF or a proteasome inhibitor, which cause increase in the expression level of endogenous Homer1a, also resulted in the reduction of Homer1c puncta. These results indicate that the accumulation of Homer1a, either exogenously expressed or endogenously induced, caused redistribution and dispersion of postsynaptic clusters of Homer1c and F-actin, suggesting an important role of Homer1a in synaptic remodeling.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • Animals
  • Brain-Derived Neurotrophic Factor / pharmacology
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cells, Cultured
  • Cysteine Endopeptidases / metabolism
  • Hippocampus / cytology
  • Hippocampus / metabolism*
  • Homer Scaffolding Proteins
  • Long-Term Potentiation / genetics
  • Multienzyme Complexes / antagonists & inhibitors
  • Multienzyme Complexes / metabolism
  • Neuronal Plasticity / genetics
  • Neurons / cytology
  • Neurons / metabolism*
  • Neuropeptides / genetics
  • Neuropeptides / metabolism*
  • Proteasome Endopeptidase Complex
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism
  • Rats
  • Seizures / genetics
  • Seizures / metabolism
  • Synaptic Membranes / genetics
  • Synaptic Membranes / metabolism*
  • Synaptic Transmission / genetics*
  • Up-Regulation / genetics

Substances

  • Actins
  • Brain-Derived Neurotrophic Factor
  • Carrier Proteins
  • Homer Scaffolding Proteins
  • Multienzyme Complexes
  • Neuropeptides
  • Protein Isoforms
  • Cysteine Endopeptidases
  • Proteasome Endopeptidase Complex