Transcription factors and nuclear receptors interact with the SWI/SNF complex through the BAF60c subunit

J Biol Chem. 2004 Apr 16;279(16):16677-86. doi: 10.1074/jbc.M312288200. Epub 2003 Dec 29.

Abstract

Transcriptional activity relies on coregulators that modify chromatin structure or serve as bridging factors between transcription factors and the basal transcription machinery. We identified a new coregulator of peroxisome proliferator-activated receptor gamma, BRG1/Brm-associated factor of 60 kDa, subunit c2 (BAF60c2), in a yeast two-hybrid screen of a human adipose tissue cDNA library. BAF60c2 represents a new isoform of BAF60c, a component of the SWI/SNF (mating type switching/sucrose non-fermenting) chromatin remodeling complex. This new isoform as well as the previously identified protein, renamed BAF60c1, is localized primarily in the cell nucleus and is expressed in a wide variety of tissues. Both BAF60c isoforms bind to several nuclear receptors and transcription factors of various families. BAF60c proteins interact in a ligand-independent manner with peroxisome proliferator-activated receptor gamma and enhance its transcriptional activity. Both isoforms are enriched in the central nervous system and also modulate the transcriptional activity of retinoic acid-related orphan receptor alpha1. In conclusion, BAF60c represents a new coregulator that constitutes an important anchoring point by which the SWI/SNF complex is recruited to nuclear receptors and other transcription factors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Chromosomal Proteins, Non-Histone
  • Humans
  • Mice
  • Molecular Sequence Data
  • Protein Binding
  • Protein Subunits
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Transcription Factors / analysis*
  • Transcription Factors / metabolism

Substances

  • Chromosomal Proteins, Non-Histone
  • Protein Subunits
  • Receptors, Cytoplasmic and Nuclear
  • SMARCD1 protein, human
  • SMARCD3 protein, human
  • Transcription Factors

Associated data

  • GENBANK/AY450430
  • GENBANK/AY450431