Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
, 36 (1), 40-5

Complete Sequencing and Characterization of 21,243 Full-Length Human cDNAs

Toshio Ota  1 Yutaka SuzukiTetsuo NishikawaTetsuji OtsukiTomoyasu SugiyamaRyotaro IrieAi WakamatsuKoji HayashiHiroyuki SatoKeiichi NagaiKouichi KimuraHiroshi MakitaMitsuo SekineMasaya ObayashiTatsunari NishiToshikazu ShibaharaToshihiro TanakaShizuko IshiiJun-ichi YamamotoKaoru SaitoYuri KawaiYuko IsonoYoshitaka NakamuraKenji NagahariKatsuhiko MurakamiTomohiro YasudaTakao IwayanagiMasako WagatsumaAkiko ShiratoriHiroaki SudoTakehiko HosoiriYoshiko KakuHiroyo KodairaHiroshi KondoMasanori SugawaraMakiko TakahashiKatsuhiro KandaTakahide YokoiTakako FuruyaEmiko KikkawaYuhi OmuraKumi AbeKumiko KamiharaNaoko KatsutaKazuomi SatoMachiko TanikawaMakoto YamazakiKen NinomiyaTadashi IshibashiHiromichi YamashitaKatsuji MurakawaKiyoshi FujimoriHiroyuki TanaiManabu KimataMotoji WatanabeSusumu HiraokaYoshiyuki ChibaShinichi IshidaYukio OnoSumiyo TakiguchiSusumu WatanabeMakoto YosidaTomoko HotutaJunko KusanoKeiichi KanehoriAsako Takahashi-FujiiHiroto HaraTomo-o TanaseYoshiko NomuraSakae TogiyaFukuyo KomaiReiko HaraKazuha TakeuchiMiho AritaNobuyuki ImoseKaoru MusashinoHisatsugu YuukiAtsushi OshimaNaokazu SasakiSatoshi AotsukaYoko YoshikawaHiroshi MatsunawaTatsuo IchiharaNamiko ShiohataSanae SanoShogo MoriyaHiroko MomiyamaNoriko SatohSachiko TakamiYuko TerashimaOsamu SuzukiSatoshi NakagawaAkihiro SenohHiroshi MizoguchiYoshihiro GotoFumio ShimizuHirokazu WakebeHaretsugu HishigakiTakeshi WatanabeAkio SugiyamaMakoto TakemotoBunsei KawakamiMasaaki YamazakiKoji WatanabeAyako KumagaiShoko ItakuraYasuhito FukuzumiYoshifumi FujimoriMegumi KomiyamaHiroyuki TashiroAkira TanigamiTsutomu FujiwaraToshihide OnoKatsue YamadaYuka FujiiKouichi OzakiMaasa HiraoYoshihiro OhmoriAyako KawabataTakeshi HikijiNaoko KobatakeHiromi InagakiYasuko IkemaSachiko OkamotoRie OkitaniTakuma KawakamiSaori NoguchiTomoko ItohKeiko ShigetaTadashi SenbaKyoka MatsumuraYoshie NakajimaTakae MizunoMisato MorinagaMasahide SasakiTakushi TogashiMasaaki OyamaHiroko HataManabu WatanabeTakami KomatsuJunko Mizushima-SuganoTadashi SatohYuko ShiraiYukiko TakahashiKiyomi NakagawaKoji OkumuraTakahiro NagaseNobuo NomuraHisashi KikuchiYasuhiko MasuhoRiu YamashitaKenta NakaiTetsushi YadaYusuke NakamuraOsamu OharaTakao IsogaiSumio Sugano
Affiliations
Comparative Study

Complete Sequencing and Characterization of 21,243 Full-Length Human cDNAs

Toshio Ota et al. Nat Genet.

Abstract

As a base for human transcriptome and functional genomics, we created the "full-length long Japan" (FLJ) collection of sequenced human cDNAs. We determined the entire sequence of 21,243 selected clones and found that 14,490 cDNAs (10,897 clusters) were unique to the FLJ collection. About half of them (5,416) seemed to be protein-coding. Of those, 1,999 clusters had not been predicted by computational methods. The distribution of GC content of nonpredicted cDNAs had a peak at approximately 58% compared with a peak at approximately 42%for predicted cDNAs. Thus, there seems to be a slight bias against GC-rich transcripts in current gene prediction procedures. The rest of the cDNAs unique to the FLJ collection (5,481) contained no obvious open reading frames (ORFs) and thus are candidate noncoding RNAs. About one-fourth of them (1,378) showed a clear pattern of splicing. The distribution of GC content of noncoding cDNAs was narrow and had a peak at approximately 42%, relatively low compared with that of protein-coding cDNAs.

Similar articles

See all similar articles

Cited by 328 PubMed Central articles

See all "Cited by" articles

Publication types

LinkOut - more resources

Feedback