The effect of dietary lysine deficiency on the immune response to Newcastle disease vaccination in chickens

Avian Dis. Oct-Dec 2003;47(4):1346-51. doi: 10.1637/7008.


The effect of lysine deficiency on chicken immune function was evaluated using broiler chickens fed a diet with lysine at 67% of the control diet (1.24% lysine). The evaluation of humoral immune function was conducted by measuring the antibody production to a live Newcastle disease virus (NDV) vaccination using the hemagglutination inhibition (HI) test and enzyme-linked immunosorbent assay (ELISA). The cellular immune function was evaluated through the use of cutaneous basophil hypersensitivity test. The antibody response to NDV vaccination was reduced in broiler chickens fed a lysine-deficient diet when measured by ELISA but not when measured by HI. The cell-mediated immune response was also reduced by lysine deficiency.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Basophils / immunology
  • Chickens*
  • Diet*
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Hemagglutination Inhibition Tests / veterinary
  • Lysine / deficiency*
  • Newcastle Disease / immunology*
  • Newcastle disease virus / immunology*
  • Reference Values
  • Vaccination / veterinary
  • Viral Vaccines*


  • Viral Vaccines
  • Lysine