Purine nucleoside phosphorylase deficiency associated with a dysplastic marrow morphology

Pediatr Res. 2004 Mar;55(3):472-7. doi: 10.1203/01.PDR.0000111286.23110.F8. Epub 2004 Jan 7.


Purine nucleoside phosphorylase (PNP) deficiency is an autosomal recessive metabolic disorder characterized by severe combined immunodeficiency and by complex neurologic symptomatology including ataxia, developmental delay, and spasticity. Herein we report severe marrow dysplasia in a patient with PNP deficiency. Drug-related marrow dysfunction was unlikely, and marrow virological studies were negative. A preleukemic myelodysplastic syndrome was also unlikely due to normal marrow CD34+ cells, colony growth in clonogenic assay of marrow mononuclear cells, apoptosis rate, and Fas expression on marrow nucleated cells, as well as morphologic improvement of the marrow dysplasia after normal red blood cell transfusion. The patient's marrow stroma showed hypersensitivity to irradiation and undetectable PNP enzyme activity similar to peripheral lymphocytes. This is the first report of PNP deficiency associated with increased lymphocyte and marrow stromal sensitivity to irradiation. We conclude that marrows from patients with PNP deficiency might have hypersensitivity to irradiation and can develop dysplastic morphology, caused either directly or indirectly by the inherited enzymatic defect.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD34 / analysis
  • Apoptosis
  • Base Sequence
  • Bone Marrow / immunology
  • Bone Marrow / pathology
  • Bone Marrow / ultrastructure
  • Bone Marrow Diseases / enzymology*
  • Child, Preschool
  • DNA Primers
  • Humans
  • Male
  • Microscopy, Electron
  • Purine-Nucleoside Phosphorylase / deficiency*
  • Purine-Nucleoside Phosphorylase / metabolism
  • fas Receptor / metabolism


  • Antigens, CD34
  • DNA Primers
  • fas Receptor
  • Purine-Nucleoside Phosphorylase