Simple fluorescence assays probing conformational changes of Escherichia coli RNA polymerase during transcription initiation

Methods Enzymol. 2003:370:598-605. doi: 10.1016/S0076-6879(03)70050-0.

Authors

Ranjan Sen¹, Dipak Dasgupta

Affiliation

¹ Laboratory of Transcription Biology, Center for DNA Fingerprinting and Diagnosis, ECIL Road, Nacharam, Hyderabad 500076, India.

PMID: 14712678
DOI: 10.1016/S0076-6879(03)70050-0

No abstract available

MeSH terms

Acrylamide / pharmacology
Anisotropy
Binding Sites
Biochemistry / methods*
DNA-Directed RNA Polymerases / chemistry*
Promoter Regions, Genetic
Protein Binding
Protein Conformation
Protein Structure, Tertiary
Spectrometry, Fluorescence / methods*
Temperature
Transcription, Genetic*

Substances

Acrylamide
DNA-Directed RNA Polymerases