Neuronal-NOS adaptor protein expression after spreading depression: implications for NO production and ischemic tolerance

J Neurochem. 2003 Dec;87(6):1368-80. doi: 10.1046/j.1471-4159.2003.02099.x.

Abstract

Cortical spreading depression (CSD) is characterized by slowly propagating waves of neuronal/astrocytic depolarization and metabolic changes, followed by a period of quiescent neuronal and electroencephalographic activity. CSD acts as a preconditioning stimulus in brain, reducing cell death when elicited up to several days prior to an ischemic insult. Precise mechanisms associated with this neuroprotection are not known, although CSD increases the expression of a number of potentially neuroprotective genes/proteins. The nitric oxide (NO) system may be of particular importance, as it is acutely activated and chronically up-regulated in cerebral cortex by CSD, and NO can ameliorate and exacerbate cell death under different conditions. Several molecules have recently been identified that modulate the production and/or cellular actions of NO, but it is not known whether their expression is altered by CSD. Therefore, the present study examined the effect of CSD on the spatiotemporal expression of PIN, CAPON, PSD-95, Mn-SOD and Cu/Zn-SOD mRNA in the rat brain. In situ hybridization using specific [35S]-labelled oligonucleotides revealed that levels of PIN mRNA were significantly increased in the cortex and claustrum ( approximately 30-180%; p </= 0.01) after 6 h and 1 and 2 days, but were again equivalent to contralateral (control) cortical values at 7, 14 and 28 days. CAPON mRNA levels were increased ( approximately 30-180%; p </= 0.05) in the ipsilateral cortical hemisphere at 6 h and 2 days post treatment, but not at the other times examined. In contrast, levels of PSD-95, Mn- and Cu/Zn-SOD mRNA were not altered at any time after CSD. These results suggest that following CSD, nNOS activity and NO levels may be tightly regulated by both transcriptional and translational alterations in a range of nNOS adaptor proteins, which may contribute to CSD-induced neuroprotection against subsequent ischemia.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing*
  • Adaptor Proteins, Vesicular Transport / metabolism*
  • Animals
  • Autoradiography
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism
  • Cerebral Cortex / anatomy & histology
  • Cerebral Cortex / drug effects
  • Cerebral Cortex / physiology*
  • Cortical Spreading Depression / drug effects
  • Cortical Spreading Depression / physiology*
  • Disks Large Homolog 4 Protein
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism
  • Dyneins / metabolism
  • Functional Laterality
  • Gene Expression Regulation / drug effects
  • Guanylate Kinases
  • In Situ Hybridization / methods
  • Intracellular Signaling Peptides and Proteins
  • Ischemia / metabolism
  • Male
  • Membrane Proteins
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism
  • Nitric Oxide / metabolism*
  • Nitric Oxide Synthase / metabolism*
  • Nitric Oxide Synthase Type I
  • Oligonucleotide Probes / metabolism
  • Potassium Chloride / pharmacology
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Superoxide Dismutase / genetics
  • Superoxide Dismutase / metabolism
  • Time Factors

Substances

  • Adaptor Proteins, Signal Transducing
  • Adaptor Proteins, Vesicular Transport
  • Carrier Proteins
  • Cell Cycle Proteins
  • Disks Large Homolog 4 Protein
  • Dlg4 protein, mouse
  • Dlg4 protein, rat
  • Drosophila Proteins
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • Microtubule-Associated Proteins
  • NOS1AP protein, rat
  • Nerve Tissue Proteins
  • Nos1ap protein, mouse
  • Oligonucleotide Probes
  • Pins protein, Drosophila
  • RNA, Messenger
  • kinesin light-chain proteins
  • postsynaptic density proteins
  • Nitric Oxide
  • Potassium Chloride
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type I
  • Nos1 protein, rat
  • Superoxide Dismutase
  • Guanylate Kinases
  • Dyneins