Coupling of caspase-9 to Apaf1 in response to loss of pRb or cytotoxic drugs is cell-type-specific

EMBO J. 2004 Jan 28;23(2):460-72. doi: 10.1038/sj.emboj.7600039. Epub 2004 Jan 8.


Inactivation of the tumor suppressor Rb in the mouse induces cell death, which depends entirely (in lens, CNS) and only partly (PNS, skeletal muscles) on Apaf1/Ced4, an apoptosomal factor thought to be required for processing procaspase-9 following mitochondrial permeabilization. Here, we report that in response to cytotoxic drugs, Apaf1(-/-) primary myoblasts but not fibroblasts undergo bona fide apoptosis. Cell demise was associated with disruption of mitochondria but not endoplasmic reticulum. Processing of procaspase-9 occurred in Apaf1(-/-) myoblasts but not fibroblasts, and ablation of Casp9 prevented drug-induced apoptosis in both cell types. Deregulation of the Rb pathway by overexpression of E2F1 also induced caspase-9-dependent, Apaf1-independent apoptosis in myoblasts. Despite its requirement for apoptosis in vitro, mutation in Casp9 abrogated cell death in the nervous system and lens but only partly in skeletal muscles of Rb-deficient embryos. In addition, developmental cell death in fetal liver and PNS was not inhibited in Casp9(-/-) embryos. Therefore, loss of pRb elicits apoptosome-dependent and apoptosome-independent cell death, and the requirement and coupling of caspase-9 to Apaf1 are both context-dependent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis*
  • Apoptotic Protease-Activating Factor 1
  • Caspase 9
  • Caspases / genetics
  • Caspases / physiology*
  • Cells, Cultured
  • Endoplasmic Reticulum / physiology
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Mice
  • Mice, Knockout
  • Mitochondria / physiology
  • Muscle, Skeletal / cytology
  • Mutation
  • Myoblasts / cytology
  • Myoblasts / drug effects
  • Myoblasts / metabolism
  • Nervous System / cytology
  • Nervous System / enzymology
  • Proteins / genetics
  • Proteins / physiology*
  • Retinoblastoma Protein / genetics
  • Retinoblastoma Protein / metabolism*
  • Signal Transduction
  • Staurosporine / toxicity


  • Apaf1 protein, mouse
  • Apoptotic Protease-Activating Factor 1
  • Proteins
  • Retinoblastoma Protein
  • Casp9 protein, mouse
  • Caspase 9
  • Caspases
  • Staurosporine