A myosin inhibitor impairs auxin-induced cell division

Protoplasma. 2003;222(3-4):193-204. doi: 10.1007/s00709-003-0025-3. Epub 2003 Dec 19.

Abstract

The role of myosins for auxin-induced cell division was probed using the inhibitor 2,3-butanedione monoxime in the tobacco cell line VBI-0, where cell elongation and division are axially aligned under the control of auxin. A morphometric analysis revealed that cell division is blocked in a dose-dependent manner, whereas cell expansion continued. In addition, the polarity of terminal cells was impaired resulting in malformed, pear-shaped cells. Early effects of the inhibitor are aberrant features of the cytoarchitecture including a block of vesicle transport, a diffuse broadening of cross walls, and the disorganization of the actin cytoskeleton. The findings are discussed with respect to a possible role of myosins as link between vesicle flow and signal control of cell division.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / drug effects
  • Actins / ultrastructure
  • Cell Division / drug effects
  • Cell Division / physiology*
  • Cell Line
  • Cytoplasm / drug effects
  • Cytoplasm / ultrastructure
  • Diacetyl / pharmacology*
  • Indoleacetic Acids / antagonists & inhibitors
  • Indoleacetic Acids / physiology
  • Myosins / antagonists & inhibitors
  • Myosins / physiology*
  • Signal Transduction
  • Tobacco / cytology*

Substances

  • Actins
  • Indoleacetic Acids
  • Myosins
  • Diacetyl