Specific oligonucleotide primers for identification of Cladophialophora carrionii, a causative agent of chromoblastomycosis

Paride Abliz; Kazutaka Fukushima; Kayoko Takizawa; Kazuko Nishimura

doi:10.1128/JCM.42.1.404-407.2004

Specific oligonucleotide primers for identification of Cladophialophora carrionii, a causative agent of chromoblastomycosis

J Clin Microbiol. 2004 Jan;42(1):404-7. doi: 10.1128/JCM.42.1.404-407.2004.

Authors

Paride Abliz¹, Kazutaka Fukushima, Kayoko Takizawa, Kazuko Nishimura

Affiliation

¹ Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba, Japan.

Abstract

Cladophialophora carrionii is one of the relatively common causative agents of chromoblastomycosis. We have developed the specific oligonucleotide primer set based on the internal transcribed spacer regions of ribosomal DNA for the rapid identification of this pathogen. PCR with this primer set amplified a 362-bp amplicon from C. carrionii strains. From other relevant dematiaceous species, including medically important dematiaceous fungi, such as Fonsecaea pedrosoi, Phialophora verrucosa, and Exophiala dermatitidis, and eight species of medically important yeasts, such as Candida albicans and Cryptococcus neoformans var. neoformans, the primer set did not produce any amplicon. PCR with this primer set may be a useful tool for the identification of C. carrionii.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromoblastomycosis / microbiology*
Cladosporium / classification
Cladosporium / genetics
Cladosporium / isolation & purification*
DNA Primers
Humans
Polymerase Chain Reaction / methods*

Substances

DNA Primers