Immunohistochemical evaluation of mononuclear infiltrates in canine lupoid onychodystrophy

Vet Pathol. 2004 Jan;41(1):37-43. doi: 10.1354/vp.41-1-37.


Claw biopsy samples of 11 dogs with lupoid onychodystrophy were evaluated. They were stained with hematoxylin and eosin and with antibodies against CD 3 as a T-cell marker, BLA 36 and HM 57 (CD 79alpha) as B-cell markers, and lysozyme, Mac 387, and major histocompatibility complex (MHC) class II as a marker for histiocytes using an immunoperoxidase and avidin-biotin technique. Inflammatory cells were counted in five high-power fields. The inflammatory infiltrate comprised predominantly B cells and T cells. Macrophages were typically only present in small numbers. CD 3, BLA 36, lysozyme, and MHC class II preserved significant antigenicity during formalin fixation and short decalcification for 24-48 hours, whereas CD 79alpha and particularly Mac 387 seemed to be more susceptible to denaturation by the decalcification process.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / immunology
  • Cell Count
  • Dog Diseases / immunology*
  • Dogs
  • Foot Diseases / immunology
  • Foot Diseases / veterinary*
  • Histiocytes / immunology
  • Hoof and Claw / immunology*
  • Hoof and Claw / pathology
  • Immunoenzyme Techniques
  • Immunohistochemistry
  • Leukocytes, Mononuclear / immunology*
  • Leukocytes, Mononuclear / pathology
  • Lupus Erythematosus, Systemic / immunology
  • Lupus Erythematosus, Systemic / veterinary*


  • Antibodies