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. 2003 Nov;9(11):1465-7.
doi: 10.3201/eid0911.020788.

Dengue-1 virus isolation during first dengue fever outbreak on Easter Island, Chile

Cecilia Perret  1 , Katia Abarca, Jimena Ovalle, Pablo Ferrer, Paula Godoy, Andrea Olea, Ximena Aguilera, Marcela Ferrés
Affiliations

Dengue-1 virus isolation during first dengue fever outbreak on Easter Island, Chile

Cecilia Perret et al. Emerg Infect Dis. 2003 Nov.

Abstract

Dengue virus was detected for the first time in Chile, in an outbreak of dengue fever on Easter Island. The virus was isolated in tissue culture and characterized by reverse transcription-polymerase chain reaction as being dengue type 1.

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Figures

Figure 1
Figure 1
Indirect immunofluorescence antibody testing with monoclonal antibodies identifying dengue-1 virus in tissue culture of Vero cells.
Figure 2
Figure 2
Visualization of reverse transcription–nested polymerase chain reaction product from 15 cultures of supernatant. DENV-1 positive samples are indicated by a 482-bp band. A) Lanes 1–5: positive culture supernatants. Lanes 6–7: negative culture supernatants. Lane 8: positive culture supernatant. Lane 9: positive DENV-1 control. Lane 10: positive DENV-2 control. Lane 11: positive DENV-3 control. Lane 12: positive DENV-4 control. Lane 13: 100-bp DNA ladder. B) Lanes 1–7: positive culture supernatants. Lane 8: negative control. Lane 9: 100-bp DNA ladder. Lane 10: positive DENV-1 control. Lane 11: positive DENV-2 control. Lane 12: positive DENV-3 control. Lane 13: positive DENV-4 control.
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