Dedifferentiation of lineage-committed cells by a small molecule

J Am Chem Soc. 2004 Jan 21;126(2):410-1. doi: 10.1021/ja037390k.

Abstract

Combinatorial libraries were screened for molecules that induce mouse myogenic lineage committed cells to dedifferentiate in vitro. A 2,6-disubstituted purine, reversine, was discovered that induces lineage reversal of C2C12 cells to become multipotent progenitor cells which can redifferentiate into osteoblasts and adipocytes. This and other such molecules are likely to provide new insights into the molecular mechanisms that control cellular dedifferentiation and may ultimately be useful to in vivo stem cell biology and therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / cytology
  • Adipocytes / drug effects
  • Animals
  • Cell Differentiation / drug effects*
  • Cell Differentiation / physiology
  • Cell Lineage
  • Mice
  • Molecular Weight
  • Morpholines / chemistry
  • Morpholines / pharmacology*
  • Myoblasts / cytology
  • Myoblasts / drug effects
  • Osteocytes / cytology
  • Osteocytes / drug effects
  • Purines / chemistry
  • Purines / pharmacology*
  • Stem Cells / cytology
  • Stem Cells / drug effects*

Substances

  • Morpholines
  • Purines
  • 2-(4-morpholinoanilino)-6-cyclohexylaminopurine