Abstract
Centromere protein F (CENP-F) expression (localization and characteristics) in relation to tumor clinicopathological parameters was immunohistochemically examined and evaluated in 47 archival biopsy specimens of buccal and gingival squamous cell carcinomas (SCC). Centromere protein F expression was detected in 79% of the samples. An increase in the labeling index (LI) with WHO grading was obtained (P < 0.05). Correlations were obtained between the CENP-F LI and tumor size (P < 0.05). Immunoelectron microscopy showed CENP-F nuclear staining as punctate or fine dots. The present study shows that CENP-F expression and detection of a more specific cell subpopulation presents a theoretical advantage for the analysis of the precise cell cycle of G2 to M cells, compared to Ki-67.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Aged
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Aged, 80 and over
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Biomarkers, Tumor / metabolism*
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Carcinoma, Squamous Cell / metabolism
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Carcinoma, Squamous Cell / mortality
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Carcinoma, Squamous Cell / pathology*
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Cell Nucleus / metabolism
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Cell Nucleus / ultrastructure
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Chromosomal Proteins, Non-Histone / metabolism*
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Cyclin D
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Cyclins / metabolism
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Female
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Gingiva / metabolism
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Gingiva / pathology*
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Gingival Neoplasms / metabolism
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Gingival Neoplasms / mortality
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Gingival Neoplasms / pathology*
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Humans
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Immunoenzyme Techniques / methods
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Ki-67 Antigen / analysis
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Male
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Microfilament Proteins / metabolism*
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Microscopy, Immunoelectron
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Middle Aged
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Mouth Mucosa / metabolism
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Mouth Mucosa / pathology*
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Neoplasm Staging
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Survival Rate
Substances
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Biomarkers, Tumor
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Chromosomal Proteins, Non-Histone
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Cyclin D
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Cyclins
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Ki-67 Antigen
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Microfilament Proteins
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centromere protein F