Immunohistochemical analysis of centromere protein F expression in buccal and gingival squamous cell carcinoma

Pathol Int. 2004 Feb;54(2):82-9. doi: 10.1111/j.1440-1827.2004.01596.x.

Abstract

Centromere protein F (CENP-F) expression (localization and characteristics) in relation to tumor clinicopathological parameters was immunohistochemically examined and evaluated in 47 archival biopsy specimens of buccal and gingival squamous cell carcinomas (SCC). Centromere protein F expression was detected in 79% of the samples. An increase in the labeling index (LI) with WHO grading was obtained (P < 0.05). Correlations were obtained between the CENP-F LI and tumor size (P < 0.05). Immunoelectron microscopy showed CENP-F nuclear staining as punctate or fine dots. The present study shows that CENP-F expression and detection of a more specific cell subpopulation presents a theoretical advantage for the analysis of the precise cell cycle of G2 to M cells, compared to Ki-67.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor / metabolism*
  • Carcinoma, Squamous Cell / metabolism
  • Carcinoma, Squamous Cell / mortality
  • Carcinoma, Squamous Cell / pathology*
  • Cell Nucleus / metabolism
  • Cell Nucleus / ultrastructure
  • Chromosomal Proteins, Non-Histone / metabolism*
  • Cyclin D
  • Cyclins / metabolism
  • Female
  • Gingiva / metabolism
  • Gingiva / pathology*
  • Gingival Neoplasms / metabolism
  • Gingival Neoplasms / mortality
  • Gingival Neoplasms / pathology*
  • Humans
  • Immunoenzyme Techniques / methods
  • Ki-67 Antigen / analysis
  • Male
  • Microfilament Proteins / metabolism*
  • Microscopy, Immunoelectron
  • Middle Aged
  • Mouth Mucosa / metabolism
  • Mouth Mucosa / pathology*
  • Neoplasm Staging
  • Survival Rate

Substances

  • Biomarkers, Tumor
  • Chromosomal Proteins, Non-Histone
  • Cyclin D
  • Cyclins
  • Ki-67 Antigen
  • Microfilament Proteins
  • centromere protein F