Contraction-induced translocation of the glucose transporter Glut4 in isolated ventricular cardiomyocytes

Biochem Biophys Res Commun. 1992 Dec 15;189(2):1207-14. doi: 10.1016/0006-291x(92)92333-s.


Field stimulation of isolated adult ventricular cardiomyocytes was used to study the effect of contractile activity on 3-O-methylglucose transport and the subcellular distribution of Glut4. Cells contracting at a frequency of 1 Hz for 30 min exhibited unaltered basal and insulin-stimulated rates of glucose transport when compared to resting cells. However, at 5 Hz 3-O-methylglucose transport increased to 224% of control after 5 min. Under these conditions insulin was unable to produce a significant additional stimulation of glucose transport. Immunoblotting with an anti-Glut4 polyclonal antibody showed that both insulin and contraction (5 Hz) increased the amount of Glut4 in a plasma membrane fraction by about 8-fold with a parallel decrease in an intracellular membrane fraction by 60-65%. These data suggest the existence of an identical insulin- and contraction-recruitable Glut4 transporter pool in cardiomyocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3-O-Methylglucose
  • Animals
  • Blotting, Western
  • Cell Membrane / metabolism
  • Cells, Cultured
  • Electric Stimulation
  • Heart / drug effects
  • Heart Ventricles
  • Insulin / pharmacology*
  • Intracellular Membranes / drug effects
  • Kinetics
  • Male
  • Membrane Proteins / isolation & purification
  • Methylglucosides / metabolism*
  • Molecular Weight
  • Monosaccharide Transport Proteins / isolation & purification
  • Monosaccharide Transport Proteins / metabolism*
  • Myocardial Contraction* / drug effects
  • Myocardium / metabolism*
  • Rats
  • Rats, Wistar
  • Subcellular Fractions


  • Insulin
  • Membrane Proteins
  • Methylglucosides
  • Monosaccharide Transport Proteins
  • 3-O-Methylglucose