Isolation and identification of C-type natriuretic peptide in human monocytic cell line, THP-1

Biochem Biophys Res Commun. 1992 Dec 15;189(2):697-704. doi: 10.1016/0006-291x(92)92257-x.

Abstract

In a survey for unknown bioactive peptides in mammalian cell lines, we isolated peptides exhibiting a strong relaxant effect on chick rectum from a phorbol ester-supplemented culture medium of the human monocytic cell line, THP-1. The peptide was deduced to be a C-terminal 29-residue peptide derived from a human C-type natriuretic peptide (CNP) precursor. CNP mRNA was also detected in the THP-1 cells, and expression of CNP gene and CNP concentration in the culture medium was found to be highly augmented by the stimulation of phorbol ester. Production of CNP in THP-1 cells suggests that CNP also functions as a local regulator in the blood cell-vascular system, although CNP has previously been recognized as a neuropeptide functioning in the central nervous system.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Atrial Natriuretic Factor / genetics
  • Atrial Natriuretic Factor / isolation & purification*
  • Atrial Natriuretic Factor / pharmacology
  • Chickens
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Humans
  • In Vitro Techniques
  • Leukemia, Monocytic, Acute
  • Molecular Sequence Data
  • Muscle Relaxation / drug effects
  • Muscle, Smooth / drug effects
  • Muscle, Smooth / physiology*
  • Protein Precursors / genetics
  • RNA, Messenger / analysis
  • RNA, Messenger / metabolism
  • Rectum / drug effects
  • Rectum / physiology
  • Tetradecanoylphorbol Acetate / pharmacology
  • Tumor Cells, Cultured

Substances

  • Protein Precursors
  • RNA, Messenger
  • Atrial Natriuretic Factor
  • Tetradecanoylphorbol Acetate