XIST unmethylated DNA fragments in male-derived plasma as a tumour marker for testicular cancer

Lancet. 2004 Jan 3;363(9402):40-2. doi: 10.1016/S0140-6736(03)15170-7.

Abstract

Testicular germ-cell tumours (TGCTs) are the most common malignant diseases among men aged 20-40 years. We developed a DNA tumour marker for TGCTs based on the unmethylated DNA profile of a neoplasm. The 5' end of the XIST gene is mainly hypomethylated in TGCTs irrespective of XIST expression. Male somatic cells, however, show complete methylation through the CpG sites, including the minimum promoter and XIST-conserved repeats. Identification of a XIST unmethylated fragment in male plasma might be diagnostic for TGCTs.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / blood
  • Chromosomes, Human, X / genetics
  • Chromosomes, Human, X / metabolism
  • DNA Methylation
  • DNA, Neoplasm / analysis
  • DNA, Neoplasm / blood
  • DNA, Neoplasm / metabolism
  • Female
  • Gene Expression Regulation, Neoplastic / genetics
  • Germinoma / blood
  • Germinoma / diagnosis*
  • Germinoma / genetics*
  • Humans
  • Male
  • Methylation
  • RNA, Long Noncoding
  • RNA, Untranslated / blood
  • RNA, Untranslated / genetics
  • RNA, Untranslated / metabolism
  • Testicular Neoplasms / blood
  • Testicular Neoplasms / diagnosis*
  • Testicular Neoplasms / genetics*

Substances

  • Biomarkers, Tumor
  • DNA, Neoplasm
  • RNA, Long Noncoding
  • RNA, Untranslated
  • XIST non-coding RNA