Effects of strength training on muscle lactate release and MCT1 and MCT4 content in healthy and type 2 diabetic humans

J Physiol. 2004 Apr 1;556(Pt 1):297-304. doi: 10.1113/jphysiol.2003.058222. Epub 2004 Jan 14.


Lactate is released from skeletal muscle in proportion to glucose uptake rates, and it leaves the cells via simple diffusion and two monocarboxylate transporter proteins, MCT1 and MCT4. In response to endurance training MCT1 - and possibly MCT4 - content in muscle increases. The MCTs have not previously been measured in patients with type 2 diabetes (Type 2), and the response to strength training is unknown. Ten Type 2 and seven healthy men (Control) strength-trained one leg (T) 3 times a week for 6 weeks while the other leg remained untrained (UT). Each session lasted no more than 30 min. After strength training, muscle biopsies were obtained and an isoglycaemic, hyperinsulinaemic clamp, combined with arterial and femoral venous catheterization of both legs, was carried out. During hyperinsulinaemia lactate release was always increased in T versus UT legs. MCT1 was lower (P<0.05) and MCT4 similar in Type 2 versus Control. With training, MCT1 content always increased, while MCT4 only increased in Control.

Conclusions: MCT1 content in skeletal muscle in Type 2 is lower compared with healthy men. Strength training increases MCT1 content in healthy men and in Type 2, thus normalizing the content in Type 2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blood Glucose / metabolism
  • Case-Control Studies
  • Cell Cycle Proteins / metabolism*
  • Diabetes Mellitus, Type 2 / blood
  • Diabetes Mellitus, Type 2 / metabolism*
  • Diabetes Mellitus, Type 2 / pathology
  • Glucose Clamp Technique
  • Humans
  • Insulin / blood
  • L-Lactate Dehydrogenase / metabolism
  • Lactic Acid / blood
  • Lactic Acid / metabolism*
  • Leg
  • Male
  • Middle Aged
  • Monocarboxylic Acid Transporters / metabolism*
  • Muscle Proteins / metabolism*
  • Muscle, Skeletal / metabolism
  • Muscle, Skeletal / physiology*
  • Oncogene Proteins / metabolism*
  • Osmolar Concentration
  • Physical Education and Training*
  • Physical Endurance*


  • Blood Glucose
  • Cell Cycle Proteins
  • Insulin
  • MCTS1 protein, human
  • Monocarboxylic Acid Transporters
  • Muscle Proteins
  • Oncogene Proteins
  • SLC16A4 protein, human
  • Lactic Acid
  • L-Lactate Dehydrogenase