The Arabidopsis homologue of Xrcc3 plays an essential role in meiosis

EMBO J. 2004 Jan 28;23(2):439-49. doi: 10.1038/sj.emboj.7600055. Epub 2004 Jan 15.

Abstract

The eukaryotic RecA homologue Rad51 is a key factor in homologous recombination and recombinational repair. Rad51-like proteins have been identified from yeast (Rad55, Rad57 and Dmc1) to vertebrates (Rad51B, Rad51C, Rad51D, Xrcc2, Xrcc3 and Dmc1). These Rad51-like proteins are all members of the genetic recombination and DNA damage repair pathways. The sequenced genome of Arabidopsis thaliana encodes putative homologues of all six vertebrate Rad51-like proteins. We have identified and characterized an Arabidopsis mutant defective for one of these, AtXRCC3, the homologue of XRCC3. atxrcc3 plants are sterile, while they have normal vegetative development. Cytological observation shows that the atxrcc3 mutation does not affect homologous chromosome synapsis, but leads to chromosome fragmentation after pachytene, thus disrupting both male and female gametogenesis. This study shows an essential role for AtXrcc3 in meiosis in plants and possibly in other higher eukaryotes. Furthermore, atxrcc3 cells and plants are hypersensitive to DNA-damaging treatments, supporting the involvement of this Arabidopsis Rad51-like protein in recombinational repair.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / anatomy & histology
  • Arabidopsis / cytology*
  • Arabidopsis / genetics
  • Arabidopsis Proteins / chemistry
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / physiology*
  • Chromosome Pairing
  • DNA Repair
  • DNA-Binding Proteins / chemistry
  • Flowers / anatomy & histology
  • Flowers / growth & development
  • Flowers / ultrastructure
  • Meiosis
  • Mitomycin / pharmacology
  • Mutagenesis, Insertional
  • Sequence Homology

Substances

  • Arabidopsis Proteins
  • At5g57450 protein, Arabidopsis
  • DNA-Binding Proteins
  • X-ray repair cross complementing protein 3
  • Mitomycin