Embryonic lethality, decreased erythropoiesis, and defective octamer-dependent promoter activation in Oct-1-deficient mice

Mol Cell Biol. 2004 Feb;24(3):1022-32. doi: 10.1128/MCB.24.3.1022-1032.2004.


Oct-1 is a sequence-specific DNA binding transcription factor that is believed to regulate a large group of tissue-specific and ubiquitous genes. Both Oct-1 and the related but tissue-restricted Oct-2 protein bind to a DNA sequence termed the octamer motif (5'-ATGCAAAT-3') with equal affinity in vitro. To address the role of Oct-1 in vivo, an Oct-1-deficient mouse strain was generated by gene targeting. Oct-1-deficient embryos died during gestation, frequently appeared anemic, and suffered from a lack of Ter-119-positive erythroid precursor cells. This defect was cell intrinsic. Fibroblasts derived from these embryos displayed a dramatic decrease in Oct-1 DNA binding activity and a lack of octamer-dependent promoter activity in transient transfection assays. Interestingly, several endogenous genes thought to be regulated by Oct-1 showed no change in expression. When crossed to Oct-2(+/-) animals, transheterozygotes were recovered at a very low frequency. These findings suggest a critical role for Oct-1 during development and a stringent gene dosage effect with Oct-2 in mediating postnatal survival.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • DNA-Binding Proteins / deficiency*
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Embryo Loss / genetics*
  • Erythropoiesis / genetics*
  • Erythropoiesis / physiology
  • Gene Targeting
  • Genes, Lethal*
  • Globins / biosynthesis
  • Globins / genetics
  • Heterozygote
  • Host Cell Factor C1
  • Mice
  • Mutation
  • Octamer Transcription Factor-1
  • Octamer Transcription Factor-2
  • Promoter Regions, Genetic*
  • Sequence Analysis, DNA
  • Transcription Factors / deficiency*
  • Transcription Factors / genetics
  • Transcription Factors / metabolism


  • DNA-Binding Proteins
  • Hcfc1 protein, mouse
  • Host Cell Factor C1
  • Octamer Transcription Factor-1
  • Octamer Transcription Factor-2
  • Pou2f1 protein, mouse
  • Pou2f2 protein, mouse
  • Transcription Factors
  • Globins