Protein profile of symbiotic bacteria Mesorhizobium loti MAFF303099 in mid-growth phase

Biosci Biotechnol Biochem. 2003 Dec;67(12):2668-73. doi: 10.1271/bbb.67.2668.


Expressed proteins in cultured symbiotic bacteria (Mesorhizobium loti MAFF303099) in the mid-growth phase were proteomically analyzed by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and capillary high-performance liquid chromatography coupled with an ion-trap mass spectrometry (MS). The genome sequence data of M. loti were used to identify the analyzed proteins. We identified 114 of the 127 proteins analyzed on 2D-PAGE gel with some microheterogenities which were caused by post-translational modifications.

MeSH terms

  • Chromatography, High Pressure Liquid
  • Electrophoresis, Gel, Two-Dimensional
  • Lotus / microbiology
  • Proteobacteria / chemistry*
  • Proteome / analysis*
  • Proteome / chemistry
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Symbiosis / physiology


  • Proteome