Endocytosis in yeast: evidence for the involvement of a small GTP-binding protein (Ypt7p)

Cell. 1992 Dec 24;71(7):1131-42. doi: 10.1016/s0092-8674(05)80062-5.


From the budding yeast S. cerevisiae, we have cloned a gene, YPT7, that encodes a GTP-binding protein belonging to the Ypt family of ras-related proteins. The 208 amino acid protein shares identical effector domain and C-terminal sequences with the mammalian Rab7 protein. YPT7 gene disruption did not impair cellular growth at temperatures ranging from 17 degrees C to 37 degrees C. ypt7 null mutants are characterized by highly fragmented vacuoles and differential defects of vacuolar protein transport and maturation. The uptake of alpha factor pheromone by wild-type and Ypt7p-deficient cells was found to be indistinguishable, but in mutant cells lacking Ypt7p, degradation of the endocytosed pheromone was severely inhibited. Our findings suggest a role of Ypt7p in protein transport between endosome-like compartments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Biological Transport
  • DNA Mutational Analysis
  • Endocytosis
  • GTP-Binding Proteins / genetics*
  • Genes, ras
  • Molecular Sequence Data
  • Proteins / metabolism
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid
  • Vacuoles / metabolism
  • rab GTP-Binding Proteins*


  • Proteins
  • Saccharomyces cerevisiae Proteins
  • GTP-Binding Proteins
  • YPT7 protein, S cerevisiae
  • rab GTP-Binding Proteins

Associated data

  • GENBANK/L05499
  • GENBANK/M87278
  • GENBANK/M94264
  • GENBANK/M97694
  • GENBANK/S72766
  • GENBANK/S72767
  • GENBANK/S72768
  • GENBANK/S72769
  • GENBANK/S72771
  • GENBANK/X68144