TAP1 mutant mice are deficient in antigen presentation, surface class I molecules, and CD4-8+ T cells

Cell. 1992 Dec 24;71(7):1205-14. doi: 10.1016/s0092-8674(05)80068-6.

Abstract

The transporter associated with the antigen processing 1 (TAP1) gene encodes a subunit for a transporter, presumed to be involved in the delivery of peptides across the endoplasmic reticulum membrane to class I molecules. We have generated mice with a disrupted TAP1 gene using embryonic stem cell technology. TAP1-deficient mice are defective in the stable assembly and intracellular transport of class I molecules and consequently show severely reduced levels of surface class I molecules. These properties are strikingly similar to those described for the TAP2 mutant cell line RMA-S. Cells from the TAP1-deficient mice are unable to present cytosolic antigens to class I-restricted cytotoxic T cells. As predicted from the near absence of class I surface expression, TAP1-deficient mice lack CD4-8+ T cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Base Sequence
  • CD4 Antigens / genetics*
  • CD8 Antigens / genetics*
  • Cell Line
  • Chimera
  • Gene Expression Regulation
  • Genes, MHC Class I / genetics*
  • Mice
  • Mice, Transgenic
  • Molecular Sequence Data
  • T-Lymphocytes / metabolism

Substances

  • CD4 Antigens
  • CD8 Antigens