Cloning and functional characterization of resistin-like molecule gamma

Biochem Biophys Res Commun. 2004 Feb 6;314(2):356-62. doi: 10.1016/j.bbrc.2003.12.100.


Resistin, a recently discovered hormone that may play a crucial role in obesity-associated diabetes, is the founding member of a novel family of cysteine-rich proteins that are secreted by specific cell types. Three other members of this family have been described to date and were termed resistin-like molecules (RELMs). Here we describe the cloning and functional characterization of RELMgamma. The mouse RELMgamma-cDNA encodes a protein of 117 amino acids that contains a signal peptide leading to secretion of the protein. By Northern blotting the RELMgamma-mRNA is detectable in bone marrow, spleen, and lung as well as in peripheral blood granulocytes. Promyelocytic HL60 cells transfected with a RELMgamma expression plasmid have an increased proliferation rate compared to mock-transfected cells and display an altered response to retinoic acid-induced granulocytic differentiation. Taken together, these data provide the first experimental evidence that RELMgamma is a secreted molecule with a restricted expression pattern that may play a role in promyelocytic differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / chemistry
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cell Differentiation
  • Cell Division
  • Cell Line
  • Cloning, Molecular
  • Cysteine / chemistry
  • DNA, Complementary / metabolism
  • Genetic Markers
  • HL-60 Cells
  • Hormones, Ectopic / chemistry*
  • Hormones, Ectopic / genetics*
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Protein Sorting Signals
  • RNA, Messenger / metabolism
  • Sequence Homology, Amino Acid
  • Time Factors
  • Tissue Distribution
  • Transfection


  • Amino Acids
  • DNA, Complementary
  • Genetic Markers
  • Hormones, Ectopic
  • Protein Sorting Signals
  • RNA, Messenger
  • resistin-like gamma, mouse
  • Cysteine