An ultrasensitive method for the detection of oligoclonal IgG bands

J Immunol Methods. 2004 Jan;284(1-2):141-5. doi: 10.1016/j.jim.2003.09.018.


We have developed an ultrasensitive isoelectrofocusing (IEF) method for the detection of oligoclonal (OGC) IgG bands in cerebrospinal fluid (CSF) and sera. In this procedure, double antibody and peroxidase immunodetection have been substituted by a single antibody labelled with alkaline phosphatase. Alkaline phosphatase immunodetection not only improves tenfold the sensitivity of the assay but also gives a sharper pattern resolution making the interpretation easier and increases the specificity of this technique. This preliminary report should be validated in a larger number of patients with and without multiple sclerosis (MS).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaline Phosphatase*
  • Humans
  • Immunoblotting
  • Immunoglobulin G / blood*
  • Immunoglobulin G / cerebrospinal fluid*
  • Immunoglobulin G / immunology
  • Isoelectric Focusing / methods*
  • Multiple Sclerosis / blood
  • Multiple Sclerosis / cerebrospinal fluid
  • Multiple Sclerosis / immunology
  • Nervous System Diseases / blood
  • Nervous System Diseases / cerebrospinal fluid
  • Nervous System Diseases / immunology*
  • Sensitivity and Specificity


  • Immunoglobulin G
  • Alkaline Phosphatase