Geminivirus DNA replication and cell cycle interactions

Vet Microbiol. 2004 Feb 4;98(2):111-9. doi: 10.1016/j.vetmic.2003.10.012.

Abstract

The Geminiviridae family includes a large number of viruses that infect plants and have a unique geminate virion particle, a single-stranded genome of approximately 2.6-3.0 kb, and replicate through a rolling-circle mechanism. Since they encode for just a few proteins (4-6 depending on the members that belong to four different genera), a rich variety of interactions has evolved between viral proteins and host factors to develop the virus replicative cycle. Among them, we have been particularly interested so far: (i). in the interference with cell cycle regulatory proteins of the retinoblastoma-related (RBR)/E2F pathway and (ii). in the interaction with host DNA replication factors necessary for the assembly of a functional replication complex at the viral origin of DNA replication during the rolling-circle stage. Yeast two-hybrid assays revealed that wheat dwarf virus RepA protein, but nor Rep protein, interacts with plant RBR protein. Interestingly, deletion of the C-terminal domain of Rep confers the truncated protein the ability to interact with RBR, suggesting that this domain may hinder the LXCXE RBR-binding motif. Secondary structure predictions support such a possibility.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Cell Cycle / physiology
  • Cell Cycle Proteins / metabolism
  • DNA Replication / physiology*
  • Geminiviridae / genetics
  • Geminiviridae / growth & development
  • Geminiviridae / metabolism
  • Geminiviridae / physiology*
  • Genome, Viral*
  • Molecular Sequence Data
  • Replication Origin
  • Retinoblastoma Protein / metabolism
  • Two-Hybrid System Techniques
  • Virus Replication / genetics*

Substances

  • Cell Cycle Proteins
  • Retinoblastoma Protein