Enhancement of RNAi Activity by Improved siRNA Duplexes

FEBS Lett. 2004 Jan 16;557(1-3):193-8. doi: 10.1016/s0014-5793(03)01492-3.


RNA interference (RNAi) is a powerful tool for suppressing the expression of a gene of interest, in which 21-25 nucleotide short interfering RNA (siRNA) duplexes homologous to the silenced gene function as sequence-specific RNAi mediators. The present study shows that newly designed siRNA duplexes, 'fork-siRNA duplexes', whose sense-stranded siRNA elements carry one to four nucleotide mismatches at the 3'-ends against the antisense-stranded siRNA elements, can enhance RNAi activity over conventional siRNA duplexes in cultured mammalian cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA Primers
  • Gene Silencing
  • HeLa Cells
  • Humans
  • Oligodeoxyribonucleotides / metabolism
  • Oligoribonucleotides / metabolism
  • Polymerase Chain Reaction / methods
  • RNA, Small Interfering / chemistry
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism*


  • DNA Primers
  • Oligodeoxyribonucleotides
  • Oligoribonucleotides
  • RNA, Small Interfering