Creatine increases IGF-I and myogenic regulatory factor mRNA in C(2)C(12) cells

FEBS Lett. 2004 Jan 16;557(1-3):243-7. doi: 10.1016/s0014-5793(03)01504-7.

Abstract

Addition of creatine to the differentiation medium of C(2)C(12) cells leads to hypertrophy of the myotubes. To investigate the implication of insulin-like growth factor I (IGF-I) and myogenic regulatory factors (MRFs) in this hypertrophy, their mRNA levels were assessed during the first 72 h of differentiation. Creatine significantly increased the IGF-I mRNA level over the whole investigated period of time, whereas the MRF mRNA levels were only augmented at precise moments, suggesting a general activation mechanism for IGF-I and a specifically regulated mechanism for MRF transcription. Our results suggest therefore that creatine-induced hypertrophy of C(2)C(12) cells is at least partially mediated by overexpression of IGF-I and MRFs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cell Differentiation / drug effects
  • Cell Line
  • Creatine / pharmacology*
  • DNA Primers
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / genetics*
  • Insulin-Like Growth Factor I / drug effects
  • Insulin-Like Growth Factor I / genetics*
  • Kinetics
  • Mice
  • Myoblasts
  • Myogenic Regulatory Factors / genetics*
  • RNA, Messenger / drug effects
  • RNA, Messenger / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • DNA Primers
  • Myogenic Regulatory Factors
  • RNA, Messenger
  • Insulin-Like Growth Factor I
  • Creatine