Imaging of adenovirus-mediated expression of human sodium iodide symporter gene by 99mTcO4 scintigraphy in mice

Nucl Med Biol. 2004 Jan;31(1):31-40. doi: 10.1016/s0969-8051(03)00100-8.


We have evaluated the feasibility of human sodium iodide symporter (hNIS) as a reporter gene in vitro and in vivo. Recombinant adenovirus encoding hNIS (Rad-hNIS) was introduced to FRO cell for 48 hours. Western blotting and 99mTcO4 uptake study revealed functional hNIS expression in the cell. Rad-hNIS was injected to BALB/c mice via tail vein. 99mTcO4 gamma scintigraphy, biodistribution study, and RT-PCR analysis demonstrated a preferential hepatic uptake of 99mTcO4, which was observed for up to one week. Thus, hNIS can be utilized as an effective reporter gene for noninvasive/repeated imaging, in combination with 99mTcO4.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / genetics*
  • Animals
  • Gene Expression Regulation / genetics
  • Genes, Reporter / genetics
  • Humans
  • Kidney / diagnostic imaging
  • Kidney / embryology
  • Kidney / metabolism*
  • Male
  • Metabolic Clearance Rate
  • Mice
  • Mice, Inbred BALB C
  • Organ Specificity
  • Radionuclide Imaging
  • Radiopharmaceuticals / pharmacokinetics
  • Recombinant Proteins / metabolism
  • Sodium Pertechnetate Tc 99m / pharmacokinetics*
  • Symporters / genetics
  • Symporters / metabolism*
  • Thyroid Gland / diagnostic imaging
  • Thyroid Gland / metabolism*
  • Tissue Distribution
  • Transformation, Genetic / genetics


  • Radiopharmaceuticals
  • Recombinant Proteins
  • Symporters
  • sodium-iodide symporter
  • Sodium Pertechnetate Tc 99m