Existence of GPR40 functioning in a human breast cancer cell line, MCF-7

Biochem Biophys Res Commun. 2004 Feb 13;314(3):805-9. doi: 10.1016/j.bbrc.2003.12.175.


GPR40, which has recently been identified as a G-protein-coupled cell-surface receptor for long-chain fatty acids, was assessed in a human breast cancer cell line (MCF-7). We detected GPR40 mRNA by RT-PCR and found that oleate and linoleate, but not palmitate or stearate, caused an increase in cellular Ca(2+) concentrations, which was partially blocked by the pertussis toxin (PTX) treatment. We examined the expression of GPR40 mRNA by quantitative RT-PCR in the relation to cell number. It was significantly increased at the beginning and at the end of cell proliferation. These results indicate the possibility that GPR40 for long-chain fatty acids may be involved in cellular function such as cell proliferation, providing a new perspective for the action of long-chain fatty acids on mammary epithelial cells.

MeSH terms

  • Breast Neoplasms / metabolism*
  • Calcium / analysis
  • Calcium / metabolism
  • Cell Division / drug effects
  • Cell Division / physiology
  • Cell Line, Tumor
  • Cytosol / metabolism
  • Dose-Response Relationship, Drug
  • Fatty Acids / antagonists & inhibitors
  • Fatty Acids / chemistry
  • Fatty Acids / pharmacology
  • Humans
  • Microscopy, Confocal
  • Pertussis Toxin / pharmacology
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Receptors, G-Protein-Coupled / biosynthesis
  • Receptors, G-Protein-Coupled / genetics
  • Receptors, G-Protein-Coupled / metabolism*
  • Signal Transduction


  • FFAR1 protein, human
  • Fatty Acids
  • RNA, Messenger
  • Receptors, G-Protein-Coupled
  • Pertussis Toxin
  • Calcium