A mechanism of induction of the mouse zinc-fingers and homeoboxes 1 (ZHX1) gene expression by interleukin-2

Biochem Biophys Res Commun. 2004 Feb 13;314(3):885-90. doi: 10.1016/j.bbrc.2003.12.162.


The effect of IL-2 on the expression of the mouse zinc-fingers and homeoboxes 1 (ZHX1) gene was investigated in a mouse cytotoxic T cell line, CTLL-2 cells. IL-2 specifically induced the expression of ZHX1 mRNA. The level of ZHX1 mRNA was decreased in the absence of IL-2. These alterations were in parallel with the status of cell proliferation. The signaling pathways involved in the induction were examined. AG-490, wortmannin, and LY294002 blocked the induction by IL-2. Nuclear run-on assays and a mRNA stability analysis revealed that the half-life of ZHX1 mRNA but not the transcription rate of the gene was increased by IL-2. Thus, we conclude that IL-2 induces the expression of the mouse ZHX1 gene in CTLL-2 cells, that both Janus kinase 3/signal transducer and activator of transcription 5 and phosphoinositide 3-kinase pathways are involved in the induction, and that the increased mRNA stability results in the induction.

MeSH terms

  • Animals
  • Blotting, Northern
  • Cell Division / drug effects
  • Cell Line
  • Enzyme Inhibitors / pharmacology
  • Gene Expression Regulation / drug effects*
  • Homeodomain Proteins / biosynthesis*
  • Homeodomain Proteins / genetics*
  • Humans
  • Interleukin-2 / genetics
  • Interleukin-2 / pharmacology*
  • Mice
  • Protein-Tyrosine Kinases / antagonists & inhibitors
  • RNA, Messenger / analysis
  • RNA, Messenger / biosynthesis
  • Recombinant Proteins / pharmacology
  • Signal Transduction / drug effects
  • T-Lymphocytes, Cytotoxic / cytology
  • T-Lymphocytes, Cytotoxic / metabolism
  • Zinc Fingers / genetics*


  • Enzyme Inhibitors
  • Homeodomain Proteins
  • Interleukin-2
  • RNA, Messenger
  • Recombinant Proteins
  • Protein-Tyrosine Kinases