Green tea extract suppresses the age-related increase in collagen crosslinking and fluorescent products in C57BL/6 mice

Int J Vitam Nutr Res. 2003 Nov;73(6):453-60. doi: 10.1024/0300-9831.73.6.453.


Collagen crosslinking during aging in part results from Maillard reaction endproducts of glucose and oxoaldehydes. Because of the tight link between oxidative and carbonyl stress, we hypothesized that natural antioxidants and "nutriceuticals" could block collagen aging in C57BL/6 mice. Six groups of young and adult mice received vitamin C, vitamin E, vitamin C&E, blueberry, green tea extract (GTE), or no treatment for a period of 14 weeks. Body weights and collagen glycation were unaltered by the treatment. However, GTE or vitamin C&E combined blocked tendon crosslinking at 10 months of age (p < 0.05, adult group). GTE also blocked fluorescent products at 385 and 440 nm (p = 0.052 and < 0.05, respectively) and tended to decrease skin pentosidine levels. These results suggest that green tea is able to delay collagen aging by an antioxidant mechanism that is in part duplicated by the combination of vitamin C and E.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aging / physiology*
  • Animals
  • Antioxidants / administration & dosage*
  • Ascorbic Acid / administration & dosage
  • Body Weight / drug effects
  • Collagen / metabolism*
  • Cross-Linking Reagents
  • Fluorescence
  • Maillard Reaction
  • Mice
  • Mice, Inbred C57BL
  • Plant Extracts / administration & dosage*
  • Random Allocation
  • Tea / chemistry*
  • Vitamin E / administration & dosage


  • Antioxidants
  • Cross-Linking Reagents
  • Plant Extracts
  • Tea
  • Vitamin E
  • Collagen
  • Ascorbic Acid