LNCIB human full-length cDNAs collection: towards a better comprehension of the human transcriptome

C R Biol. Oct-Nov 2003;326(10-11):967-70. doi: 10.1016/j.crvi.2003.09.029.

Abstract

LNCIB has been producing a variety of human full-length-enriched, normalized and subtracted cDNA libraries from various cell lines and tissues in different developmental stages by using the CAP-Trapper method. By sequencing 23000 clones of these libraries we identified a pool of about 5800 good quality unique cDNAs. After BLAST analysis on Human RefSeq/Unigene databases, 1717 of these sequences remained with no or poor annotation. We show that cross-species comparative BLAST resulted as a valid tool for the annotation of orthologous genes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Databases, Factual
  • Gene Library*
  • Genome, Human*
  • Humans
  • Transcription, Genetic*