In vivo filtering of in vitro expression data reveals MyoD targets

C R Biol. Oct-Nov 2003;326(10-11):1049-65. doi: 10.1016/j.crvi.2003.09.035.

Abstract

A published set of downstream targets of MyoD defined in a well-controlled in vitro experiment was filtered for relevance to muscle regeneration using a 27-time-point in vivo murine regeneration series. Using interactive hierarchical and Bayes soft clustering, only a minority of the targets defined in vitro can be confirmed in vivo (approximately 50% of induced transcripts, and none of repressed transcripts). This approach provided strong support that 18 targets including of MyoD are biologically relevant during myoblast differentiation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cluster Analysis
  • Gene Expression Regulation
  • Mice
  • Muscle, Skeletal / physiology*
  • MyoD Protein / genetics
  • MyoD Protein / physiology*
  • Regeneration*

Substances

  • MyoD Protein